Anti-Surfactant Protein A抗体[6F10] (ab51891)

概述

  • 产品名称Anti-Surfactant Protein A抗体[6F10]
    参阅全部 Surfactant Protein A 一抗
  • 描述
    小鼠单克隆抗体[6F10] to Surfactant Protein A
  • 特异性ab51891 is specific for human pulmonary Surfactant Protein A
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Native Surfactant Protein A purified from bronchioaveolar lavage from proteinosis patients.

  • 阳性对照
    • Human lung tissue

性能

应用

Our Abpromise guarantee covers the use of ab51891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21421565
WB 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/16000.
IHC-P Use at an assay dependent concentration.

靶标

  • 细胞定位Secreted protein; extracellular space.
  • 数据库链接
  • 别名
    • 35 kDa pulmonary surfactant-associated protein antibody
    • Alveolar proteinosis protein antibody
    • COLEC4 antibody
    • Collectin 4 antibody
    • PSAP antibody
    • PSPA antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant-associated protein A1 antibody
    • SFTP1 antibody
    • SFTPA antibody
    • SFTPA1 antibody
    • SFTPA1B antibody
    • SP A antibody
    • SP A1 antibody
    see all

Anti-Surfactant Protein A antibody [6F10] 图像

  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
  • All lanes : Anti-Surfactant Protein A antibody [6F10] (ab51891) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

Anti-Surfactant Protein A antibody [6F10] (ab51891)参考文献

This product has been referenced in:
  • Somarajan SR  et al. Annexin A2 mediates Mycoplasma pneumoniae community-acquired respiratory distress syndrome toxin binding to eukaryotic cells. MBio 5:N/A (2014). WB ; Human . Read more (PubMed: 25139904) »
  • Zhou J  et al. Biological features of novel avian influenza A (H7N9) virus. Nature 499:500-3 (2013). Read more (PubMed: 23823727) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (lung)
Loading amount 25 µg
Specification lung
Gel Running Conditions Non-reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Mun-Suk Park

Verified customer

提交于 Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"