The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 32 kDa.
Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed
lipoprotein that has been shown (by Western blot and Northern blot) to be
expressed in the European B. burgdorferi strain B29, but not in the
American strain B31. Cell envelope proteins of bacterial pathogens play
important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb.
Western blot - Anti-Surface Lipoprotein p27 antibody (ab106086)
All lanes : Anti-Surface Lipoprotein p27 antibody (ab106086) at 1/1000 dilution
Lane 1 : MBP-Surface Lipoprotein p27 fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary All lanes : Goat-anti-Rabbit HRP at 1/40000 dilution