概述

  • 产品名称Anti-Surf1抗体[21H2BG4]
    参阅全部 Surf1 一抗
  • 描述
    小鼠单克隆抗体[21H2BG4] to Surf1
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human Surf1

  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probably involved in the biogenesis of the COX complex.
  • 疾病相关Defects in SURF1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency.
  • 序列相似性Belongs to the SURF1 family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OTTHUMP00000022473 antibody
    • OTTHUMP00000022474 antibody
    • SURF 1 antibody
    • SURF-1 antibody
    • Surf1 antibody
    • SURF1_HUMAN antibody
    • Surfeit 1 antibody
    • Surfeit locus protein 1 antibody
    see all

Anti-Surf1 antibody [21H2BG4] 图像

  • Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Surf1 antibody [21H2BG4] (ab110256)参考文献

This product has been referenced in:
  • Echaniz-Laguna A  et al. SURF1 deficiency causes demyelinating Charcot-Marie-Tooth disease. Neurology 81:1523-30 (2013). WB ; Human . Read more (PubMed: 24027061) »
  • Lopes Costa A  et al. Beneficial effects of resveratrol on respiratory chain defects in patients' fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 24365713) »

See all 4 Publications for this product

Product Wall

Merci pour votre émail. Malheureusement nous n'avons pas des échantillons de nos produits.Je ne peux donc pas vous en envoyer un.

Je peux par contre vous rassurer, que nous garantissons nos produits de fonctionner tel que d&eacu...

Read More

Merci pour votre émail.

Je suis désolée d'entendre que vous avez des problèmes avec le stock d'ab110256. Normalement un anticorps peux se garder un ans à 4°C sans problème. Est-ce que vous avez v&...

Read More

Merci d'avoir pris le temps de remplir aussi un questionnaire pour le ab110256.

Voici aussi les suggestions pour cet trouver une explication pour le poids moléculaire observé avec cet anticorps:

1.) Comme pour le ab119...

Read More

Je suis désolée d'apprendre que ces anticorps ne vous donnent pas de résultats satisfaisants en Western blot - c.à.d. une bande à environ 42kD pour SURF1 et à environ 24kD pour mtTFA).
Nous n'avons pas eu des autres retours négatives et n'avons pa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"