概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to SUPT5H
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 981-1087 of Human SUPT5H (AAH24203) with a proprietary tag.

  • 阳性对照
    • Partial tagged recombinant Human SUPT5H protein (Immunogen), HeLa nuclear extract and HeLa cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 121 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the SPT5 family.
    Contains 5 KOW domains.
  • 翻译后修饰
    Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
    Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DRB sensitivity inducing factor large subunit antibody
    • DRB sensitivity-inducing factor 160 kDa subunit antibody
    • DRB sensitivity-inducing factor large subunit antibody
    • DSIF large subunit antibody
    • DSIF p160 antibody
    • FLJ34157 antibody
    • hSPT 5 antibody
    • hSPT5 antibody
    • SPT 5 antibody
    • SPT 5H antibody
    • SPT5 antibody
    • SPT5H antibody
    • SPT5H_HUMAN antibody
    • Suppressor of Ty 5 homolog antibody
    • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    • SUPT 5H antibody
    • supt5h antibody
    • Tat cotransactivator 1 protein antibody
    • Tat CT1 antibody
    • Tat CT1 protein antibody
    • Tat-cotransactivator 1 protein antibody
    • Tat-CT1 protein antibody
    • Transcription elongation factor SPT 5 antibody
    • Transcription elongation factor spt5 antibody
    • Transcription factor Tat CT1 antibody
    see all

图片

  • Anti-SUPT5H antibody (ab89219) at 5 µg/ml + Partial tagged recombinant Human SUPT5H protein at 0.2 µg

    Predicted band size : 121 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-SUPT5H antibody (ab89219) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • ab89219, at 10 µg/ml, staining SUPT5H in HeLa cells by Immunofluorescence.

文献

ab89219 has not yet been referenced specifically in any publications.

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