概述

  • 产品名称Anti-SUPT5H抗体
    参阅全部 SUPT5H 一抗
  • 描述
    兔多克隆抗体to SUPT5H
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 396 - 445 (FEDQPEGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG) of Human SUPT5H (NP_003160)

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab83974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the SPT5 family.
    Contains 5 KOW domains.
  • 翻译后修饰Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
    Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DRB sensitivity inducing factor large subunit antibody
    • DRB sensitivity-inducing factor 160 kDa subunit antibody
    • DRB sensitivity-inducing factor large subunit antibody
    • DSIF large subunit antibody
    • DSIF p160 antibody
    • FLJ34157 antibody
    • hSPT 5 antibody
    • hSPT5 antibody
    • SPT 5 antibody
    • SPT 5H antibody
    • SPT5 antibody
    • SPT5H antibody
    • SPT5H_HUMAN antibody
    • Suppressor of Ty 5 homolog antibody
    • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
    • SUPT 5H antibody
    • supt5h antibody
    • Tat cotransactivator 1 protein antibody
    • Tat CT1 antibody
    • Tat CT1 protein antibody
    • Tat-cotransactivator 1 protein antibody
    • Tat-CT1 protein antibody
    • Transcription elongation factor SPT 5 antibody
    • Transcription elongation factor spt5 antibody
    • Transcription factor Tat CT1 antibody
    see all

Anti-SUPT5H antibody 图像

  • Anti-SUPT5H antibody (ab83974) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 121 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

Anti-SUPT5H antibody (ab83974)参考文献

ab83974 has not yet been referenced specifically in any publications.

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