概述

  • 产品名称Anti-SUPT16H抗体
    参阅全部 SUPT16H 一抗
  • 描述
    兔多克隆抗体to SUPT16H
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: IIFMASKKKV EFLKQIANTK GNENANGAPA ITLLIREKNE SNKSSFDKMI, corresponding to N terminal amino acids 73-122 of Human SUPT16H

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab56212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the peptidase M24 family. SPT16 subfamily.
  • 结构域The Glu-rich acidic region in C-terminus is essential for FACT activity.
  • 翻译后修饰ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
  • 细胞定位Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC68 antibody
    • Chromatin specific transcription elongation factor 140 kDa subunit antibody
    • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SPT16 antibody
    • FACT 140 kDa subunit antibody
    • FACT antibody
    • FACT complex subunit SPT16 antibody
    • FACTp140 antibody
    • FLJ10857 antibody
    • FLJ14010 antibody
    • hSPT16 antibody
    • SP16H_HUMAN antibody
    • Suppressor of Ty 16 homolog antibody
    • Supt16h antibody
    see all

Anti-SUPT16H antibody 图像

Anti-SUPT16H antibody (ab56212)参考文献

ab56212 has not yet been referenced specifically in any publications.

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