概述

  • 产品名称Anti-SUPT16H抗体
    参阅全部 SUPT16H 一抗
  • 描述
    兔多克隆抗体to SUPT16H
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 321-640 of Human SUPT16H.

  • 阳性对照
    • Human Testis tissue

性能

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应用

Our Abpromise guarantee covers the use of ab117081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 120 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the peptidase M24 family. SPT16 subfamily.
  • 结构域The Glu-rich acidic region in C-terminus is essential for FACT activity.
  • 翻译后修饰ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
  • 细胞定位Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC68 antibody
    • Chromatin specific transcription elongation factor 140 kDa subunit antibody
    • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SPT16 antibody
    • FACT 140 kDa subunit antibody
    • FACT antibody
    • FACT complex subunit SPT16 antibody
    • FACTp140 antibody
    • FLJ10857 antibody
    • FLJ14010 antibody
    • hSPT16 antibody
    • SP16H_HUMAN antibody
    • Suppressor of Ty 16 homolog antibody
    • Supt16h antibody
    see all

Anti-SUPT16H antibody 图像

  • Human Testis (formalin-fixed, paraffin-embedded) stained with ab117081 followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-SUPT16H antibody (ab117081)参考文献

ab117081 has not yet been referenced specifically in any publications.

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