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Our Abpromise guarantee covers the use of ab54350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.|
|Dot blot||Use at an assay dependent dilution.|
The immunogen peptide in this image was tagged. Secondary antibody: Sheep Anti Mouse IgG (Original Conc.; Antibody:0.63mg/ml, HRP: 0.78mg/ml) Immunogen amount: 25ng/lane. MW of Tag: approx. 20kDa.
Antibody only tested on recombinant fragment as immunogen. We have no data on detection of endogenous protein.