Superoxide Dismutase Activity Assay试剂盒(Colorimetric) (ab65354)

概述

  • 产品名称Superoxide Dismutase Activity Assay试剂盒(Colorimetric)
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Enzyme activity
  • 检测时间
    0h 30m
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human, Pig
    预测可用于: all Mammals
  • 产品概述

    Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
  • 细胞定位Cytoplasmic
  • 别名
    • ALS
    • ALS1
    • Cu /Zn superoxide dismutase
    • Cu/Zn superoxide dismutase
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase cystolic
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab65354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Superoxide Dismutase Activity Assay Kit (Colorimetric) 图像

  • Superoxide dismutase measured in biofluids at various dilutions

  • Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)

  • Principle of Superoxide Dismutase Assay.

实验方案

Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)参考文献

This product has been referenced in:
  • Chade AR  et al. Disparate effects of single endothelin-A and -B receptor blocker therapy on the progression of renal injury in advanced renovascular disease. Kidney Int 85:833-44 (2014). Read more (PubMed: 24352153) »
  • Choudhery MS  et al. Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation. J Transl Med 12:8 (2014). Read more (PubMed: 24397850) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us.

I would not suggest this, as we store this product at 4C ourselves. We haven't tried to store this productat -20C, and we do not know if the product is suitable to be stored at lower temperatures. Please be aware...

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The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Bla...

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Thank you for contacting us.

I apologize for the problems you had with accessing our website.

As you had requested, I have attached the SDS for the 3 of the kits you mentioned.

The links to the online SDSpages are also: ...

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To our knowledge, ab65354 has not been tested specifically for use with drosophila samples. This assay, however, is not species specific. So long as the SOD present in your drosophila samples is inhibiting the red...

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Thank you for your enquiry.

I appreciate your patience. It has taken some time to confirm details with the laboratories.

This kit is not originally designed to be run with a standard curve each time. However, if you wish to run a stan...

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I can confirm that there is nothing isoform-specific in the kit principle. ab65354 measures the oxygen anion created by any of the SOD enzymes.

The minimum mass of tissue to use with this assay is 10mg.

Thank you for contacting us.

This kit has been tested in human samples only, so it may work for prokaryotic cells but we have no data on testing these types of samples.

I hope this information is helpful to you. Please do not hesitate...

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We would recommend using at least 10 mg of tissue with the kit ab65354.

We recommend use of ab112193 SOD protein as standard for this kit.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"