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Our Abpromise guarantee covers the use of ab16963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 1 - 2 µg/ml.|
ab16963 staining Superoxide Dismutse 4 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
Immunoprecipitation analysis of HeLa cell lysates using ab16963.
Lane 1- Input
Lane 2- IP
Western analysis was conducted using a polyclonal SOD4 antibody (ab16834)
ab16963 has not yet been referenced specifically in any publications.
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