Anti-Superoxide Dismutase 4抗体[2A1] (ab16963)

概述

  • 产品名称Anti-Superoxide Dismutase 4抗体[2A1]
    参阅全部 Superoxide Dismutase 4 一抗
  • 描述
    小鼠单克隆抗体[2A1] to Superoxide Dismutase 4
  • 经测试应用适用于: ICC/IF, ELISA, IPmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab16963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 1 - 2 µg/ml.

靶标

  • 功能Delivers copper to copper zinc superoxide dismutase (SOD1).
  • 组织特异性Ubiquitous.
  • 序列相似性In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.
    Contains 1 HMA domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCS antibody
    • CCS_HUMAN antibody
    • Copper chaperone for superoxide dismutase antibody
    • MGC138260 antibody
    • SOD 4 antibody
    • SOD4 antibody
    • Superoxide dismutase copper chaperone antibody
    see all

Anti-Superoxide Dismutase 4 antibody [2A1] 图像

  • ab16963 staining Superoxide Dismutse 4 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Immunoprecipitation analysis of HeLa cell lysates using ab16963.
    Lane 1- Input
    Lane 2- IP

    Western analysis was conducted using a polyclonal SOD4 antibody (ab16834)

Anti-Superoxide Dismutase 4 antibody [2A1] (ab16963)参考文献

ab16963 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"