RabMAb

Anti-Superoxide Dismutase 1抗体[EPR1726] (ab79390)

概述

  • 产品名称Anti-Superoxide Dismutase 1抗体[EPR1726]
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    兔单克隆抗体[EPR1726] to Superoxide Dismutase 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Superoxide Dismutase 1 aa 50-150.
    Database link: P00441
    (Peptide available as ab195608)

  • 阳性对照
    • WB: HeLa, MCF7, Jurkat and HT29 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab79390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

For unpurified, use 1/100000 - 1/200000.

靶标

  • 功能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Anti-Superoxide Dismutase 1 antibody [EPR1726] 图像

  • All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT-29 cell lysate
    Lane 5 : T47-D cell lysate
    Lane 6 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 16 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/200000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa

Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)参考文献

This product has been referenced in:
  • Pehar M  et al. Mitochondria-targeted catalase reverts the neurotoxicity of hSOD1G?³A astrocytes without extending the survival of ALS-linked mutant hSOD1 mice. PLoS One 9:e103438 (2014). WB . Read more (PubMed: 25054289) »
  • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . Read more (PubMed: 25133973) »

See all 2 Publications for this product

Product Wall

Unfortunately, we don't have data regarding the amount of SOD1 that can be pulled down via an IP with this antibody.
We used our standard IP protocol which can be found at the URL below:
http://www.epitomics.com/products/support/ip


Our cross reactivity recommendations for 1926-1 and 2582-1 are based off of Western blot analysis only. We do not recommend either product for detection of mouse SOD1 as we did not obtain any staining in WB with 2582-1, and we obtained non-specifi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"