Anti-Superoxide Dismutase 1抗体(ab62800)

概述

  • 产品名称Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    山羊多克隆抗体to Superoxide Dismutase 1
  • 经测试应用适用于: Sandwich ELISA, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit, Goat, Horse, Cow, Dog, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Deer, Marmoset (common), Orangutan
  • 免疫原

    Synthetic peptide:

    C-SRKHGGPKDEERH

    , corresponding to internal sequence amino acids 69-81 of Human Superoxide Dismutase 1

  • 阳性对照
    • Mouse brain lysate or rat spinal cord lysate.

性能

应用

Our Abpromise guarantee covers the use of ab62800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. increasing amount of recombinant SOD1 captured by a rabbit antibody. ab62800 used as reporter antibody.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
ELISA 1/8000. Peptide ELISA
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Anti-Superoxide Dismutase 1 antibody 图像

  • Anti-Superoxide Dismutase 1 antibody (ab62800) at 1/0.02 dilution + Mouse Brain lysate (35µg protein in RIPA buffer).

    Predicted band size : 16 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • ab62800 (1/200) staining Superoxide Dismutase 1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5%Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus. Please refer to Abreview for further experimental details.

    See Abreview

Anti-Superoxide Dismutase 1 antibody (ab62800)参考文献

This product has been referenced in:
  • Ganapathy E  et al. D-4F, an apoA-I mimetic peptide, inhibits proliferation and tumorigenicity of epithelial ovarian cancer cells by upregulating the antioxidant enzyme MnSOD. Int J Cancer 130:1071-81 (2012). WB ; Mouse . Read more (PubMed: 21425255) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Jan 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"