Anti-Superoxide Dismutase 1抗体(ab52950)


  • 产品名称Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    兔多克隆抗体to Superoxide Dismutase 1
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Superoxide Dismutase 1

  • 阳性对照
    • Jurkat cell lysate, human placenta and HeLa cells.



Our Abpromise guarantee covers the use of ab52950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/20.
IHC-P 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • 疾病相关Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • 序列相似性Belongs to the Cu-Zn superoxide dismutase family.
    • 翻译后修饰Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • 细胞定位Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Anti-Superoxide Dismutase 1 antibody 图像

    • Anti-Superoxide Dismutase 1 antibody (ab52950) at 1/5000 dilution + Jurkat cell lysate at 10 µg

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
    • ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in human placenta by Immunohistochemistry, Paraffin embedded tissue.
    • ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in HeLa cells by Immunofluorescence.

    Anti-Superoxide Dismutase 1 antibody (ab52950)参考文献

    This product has been referenced in:
    • Cacabelos D  et al. Early and gender-specific differences in spinal cord mitochondrial function and oxidative stress markers in a mouse model of ALS. Acta Neuropathol Commun 4:3 (2016). WB . Read more (PubMed: 26757991) »
    • Qi Y  et al. PGC-1a Silencing Compounds the Perturbation of Mitochondrial Function Caused by Mutant SOD1 in Skeletal Muscle of ALS Mouse Model. Front Aging Neurosci 7:204 (2015). WB ; Mouse . Read more (PubMed: 26539112) »

    See all 7 Publications for this product

    Product Wall

    Our cross reactivity recommendations for 1926-1 and 2582-1 are based off of Western blot analysis only. We do not recommend either product for detection of mouse SOD1 as we did not obtain any staining in WB with 2582-1, and we obtained non-specifi...

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    Vielen Dank für Ihre Antwort.

    Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu...

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    Vielen Dank für Ihre Anfrage.
    Ich freue mich Ihnen mitteilen zu können, dass beide Lots eine Konzentration von 0.263 mg/mL haben.
    Ich möchte auch darauf hinweisen, dass diese scheinbar niedrige Konzentration völlig ausreichend ist. Dieser Ant...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
    Loading amount 15 µg
    Specification 1 EBNA, 2 HeLa
    Gel Running Conditions Reduced Denaturing
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%

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    提交于 Dec 16 2008