Anti-Superoxide Dismutase 1抗体(ab45777)

概述

  • 产品名称
    Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    兔多克隆抗体to Superoxide Dismutase 1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Superoxide Dismutase 1.

    (Peptides available as ab46993).

  • 阳性对照
    • ab45777 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab45777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).Can be blocked with Human Superoxide Dismutase 1 peptide (ab46993).

靶标

  • 功能
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性
    Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

图片

  • ICC/IF image of ab45777 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45777, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab45777) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from human breast cancer tissue
    Lane 2 : Whole tissue lysate prepared from human breast cancer tissue

    Lysates/proteins at 8 µg per lane.

    Secondary
    HRP conjugated goat polyclonal to rabbit IgG at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size : 16 kDa


    Exposure time : 15 minutes

    This image is courtesy of an Abreview submitted by Dr Lay Harn Gam

    See Abreview

  • ab45777 (1/2000) staining SOD1 in paraffin-embedded human breast carcinoma sections. Tissues underwent formaldehyde fixation, heat-mediated antigen retrieval and peroxidase-blocking. Secondary antibody was a goat anti-rabbit IgG conjugated to HRP. For further experimental details please refer to Abreview.

    See Abreview

文献

ab45777 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Carcinoma)
Specification
Breast Carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Breast cancer)
Loading amount
8 µg
Specification
Breast cancer
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Lay Harn Gam

Verified customer

提交于 Mar 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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