Anti-Superoxide Dismutase 1抗体(ab45777)

概述

  • 产品名称Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    兔多克隆抗体to Superoxide Dismutase 1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Superoxide Dismutase 1.

    (Peptides available as ab46993).

  • 阳性对照
    • ab45777 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab45777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).Can be blocked with Human Superoxide Dismutase 1 peptide (ab46993).

靶标

  • 功能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Anti-Superoxide Dismutase 1 antibody 图像

  • ICC/IF image of ab45777 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45777, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab45777) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from human breast cancer tissue
    Lane 2 : Whole tissue lysate prepared from human breast cancer tissue

    Lysates/proteins at 8 µg per lane.

    Secondary
    HRP conjugated goat polyclonal to rabbit IgG at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size : 16 kDa


    Exposure time : 15 minutes

    This image is courtesy of an Abreview submitted by Dr Lay Harn Gam

    See Abreview

  • ab45777 (1/2000) staining SOD1 in paraffin-embedded human breast carcinoma sections. Tissues underwent formaldehyde fixation, heat-mediated antigen retrieval and peroxidase-blocking. Secondary antibody was a goat anti-rabbit IgG conjugated to HRP. For further experimental details please refer to Abreview.

    See Abreview

Anti-Superoxide Dismutase 1 antibody (ab45777)参考文献

ab45777 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast Carcinoma)
Specification Breast Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Breast cancer)
Loading amount 8 µg
Specification Breast cancer
Gel Running Conditions Reduced Denaturing (15)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Lay Harn Gam

Verified customer

提交于 Mar 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"