概述

  • 产品名称Anti-SUN1抗体
    参阅全部 SUN1 一抗
  • 描述
    兔多克隆抗体to SUN1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse SUN1.

  • 阳性对照
    • WB: Mouse and rat embryonic brain E14, E16 and E18 tissue lysates. ICC/IF: HepG2 cells.

性能

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应用

Our Abpromise guarantee covers the use of ab103021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 102 kDa). Abcam recommends using milk as the blocking agent - 3%

靶标

  • 功能Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
  • 序列相似性Contains 1 SUN domain.
  • 结构域The SUN domain may play a role in the nuclear anchoring and/or migration.
  • 细胞定位Nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein unc-84 homolog A antibody
    • Sad1 and UNC84 domain containing 1 antibody
    • Sad1/unc 84 protein like 1 antibody
    • Sad1/unc-84 protein-like 1 antibody
    • Sun domain-containing protein 1 antibody
    • Sun1 antibody
    • SUN1_HUMAN antibody
    • UNC 84 homolog A (C elegans) antibody
    • UNC 84A antibody
    • UNC84 A antibody
    • UNC84 antibody
    • UNC84 homolog A (C elegans) antibody
    • UNC84A antibody
    see all

Anti-SUN1 antibody 图像

  • ICC/IF image of ab103021 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103021, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

  • All lanes : Anti-SUN1 antibody (ab103021) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E16 Mouse Embryo Brain Tissue Lysate
    Lane 3 : E18 Mouse Embryo Brain Tissue Lysate
    Lane 4 : E14 Rat Embryo Brain Tissue Lysate
    Lane 5 : E16 Rat Embryo Brain Tissue Lysate
    Lane 6 : E18 Rat Embryo Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,24 kDa,64 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab103021 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-SUN1 antibody (ab103021)参考文献

ab103021 has not yet been referenced specifically in any publications.

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