1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Is unsuitable for Flow Cyt or IP.
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.
Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-Sumo 2+3 antibody [EPR4602] (ab109005)
All lanes : Anti-Sumo 2+3 antibody [EPR4602] (ab109005) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : 293T cell lysate Lane 4 : SW480 cell lysate Lane 5 : HL60 cell lysate