概述

  • 产品名称Anti-Sumo 2+3抗体[EPR4602]
    参阅全部 Sumo 2+3 一抗
  • 描述
    兔单克隆抗体[EPR4602] to Sumo 2+3
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Sumo 2+3 aa 1 to the C-terminus.

  • 阳性对照
    • HeLa, Jurkat, 293T, SW480 and HL60 cell lysates; Human colonic and hepatocellular carcinoma tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Sumo 2+3 antibody (Alexa Fluor® 488) [EPR4602] (ab196330)
    Anti-Sumo 2+3 antibody (Alexa Fluor® 647) [EPR4602] (ab196578)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR4602
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/400.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 相关性SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
    • 细胞定位Cytoplasmic (SUMO3) and Nuclear (SUMO2)
    • 数据库链接
    • 别名
      • HSMT3 antibody
      • MGC117191 antibody
      • OTTHUMP00000115275 antibody
      • OTTHUMP00000115276 antibody
      • OTTHUMP00000115277 antibody
      • Sentrin 2 antibody
      • Small ubiquitin like modifier 2 antibody
      • Small ubiquitin related modifier 2 antibody
      • small ubiquitin-like modifier 3 antibody
      • small ubiquitin-related modifier 3 antibody
      • SMT3 homolog 1 antibody
      • SMT3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 2 antibody
      • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
      • SMT3 suppressor of mif two 3 homolog 3 antibody
      • SMT3A antibody
      • SMT3B antibody
      • SMT3H1 antibody
      • SMT3H2 antibody
      • Sumo2 antibody
      • Sumo3 antibody
      • Ubiquitin like protein SMT3A antibody
      • Ubiquitin like protein SMT3B antibody
      see all

    Anti-Sumo 2+3 antibody [EPR4602] 图像

    • Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Sumo 2+3 antibody [EPR4602] (ab109005) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SW480 cell lysate
      Lane 5 : HL60 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
    • ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-Sumo 2+3 antibody [EPR4602] (ab109005)参考文献

    This product has been referenced in:
    • Huang CJ  et al. The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes. Sci Rep 5:15600 (2015). Mouse . Read more (PubMed: 26493771) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109005.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"