Anti-Sumo 2+3抗体[AT10F1] (ab104123)

概述

  • 产品名称Anti-Sumo 2+3抗体[AT10F1]
    参阅全部 Sumo 2+3 一抗
  • 描述
    小鼠单克隆抗体[AT10F1] to Sumo 2+3
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Recombinant Human Sumo 2 (1-93aa; MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGG) purified from E. coli.

  • 阳性对照
    • HeLa cell lysate; Recombinant Sumo 2 or Sumo 3 protein; HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号AT10F1
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab104123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500. Predicted molecular weight: 12 kDa.
ELISA 1/250.
ICC/IF 1/250 - 1/500.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • 细胞定位Cytoplasmic (SUMO3) and Nuclear (SUMO2)
  • 数据库链接
  • 别名
    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    see all

Anti-Sumo 2+3 antibody [AT10F1] 图像

  • All lanes : Anti-Sumo 2+3 antibody [AT10F1] (ab104123) at 1/500 dilution

    Lane 1 : Hela cell lysate at 35 µg
    Lane 2 : Recombinant Sumo 2 at 0.01 µg
    Lane 3 : Recombinant Sumo 3 at 0.01 µg

    Secondary
    Goat anti-mouse HRP conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
  • Immunofluorescence staining of Sumo 2+3 in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104123 (1/500) with Texas Red (Red).
  • ab104123 staining Sumo 2+3 in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.

Anti-Sumo 2+3 antibody [AT10F1] (ab104123)参考文献

ab104123 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104123.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"