Anti-Sumo 2+3抗体[8A2] - ChIP Grade (ab81371)

概述

  • 产品名称Anti-Sumo 2+3抗体[8A2] - ChIP Grade
    参阅全部 Sumo 2+3 一抗
  • 描述
    小鼠单克隆抗体[8A2] to Sumo 2+3 - ChIP Grade
  • 特异性This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1.
  • 经测试应用适用于: WB, ICC/IF, IP, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Sumo 2 protein (Human)

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab81371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 12 kDa.
ICC/IF 1/2000.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • 细胞定位Cytoplasmic (SUMO3) and Nuclear (SUMO2)
  • 数据库链接
  • 别名
    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin related modifier 2 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    see all

Anti-Sumo 2+3 antibody [8A2] - ChIP Grade 图像

  • Anti-Sumo 2+3 antibody [8A2] - ChIP Grade (ab81371) at 1/2000 dilution + HeLa cell lysate

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Ab81371 (1:2000) staining human Sumo 2 + 3 in HeLa cells by immunofluorescence.
  • IHC image of ab81371 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81371, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Sumo 2+3 antibody [8A2] - ChIP Grade (ab81371)参考文献

This product has been referenced in:
  • Conn KL  et al. Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response. J Virol 90:4807-26 (2016). Read more (PubMed: 26937035) »
  • Hochrainer K  et al. SUMO2/3 is associated with ubiquitinated protein aggregates in the mouse neocortex after middle cerebral artery occlusion. J Cereb Blood Flow Metab 35:1-5 (2015). Read more (PubMed: 25352045) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (breast cancer cell lines)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification breast cancer cell lines
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 01 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 0.1% · Temperature: 25°C
Sample Rat Cell (Glial cells)
Specification Glial cells
Permeabilization Yes - 0.01% Saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 10 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Cell lysate - whole cell (Mixed glial cells)
Specification Mixed glial cells
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2014

Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Xenopus egg extract)
Loading amount 40 µg
Specification Xenopus egg extract
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Andrew Lane

Verified customer

提交于 Apr 11 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Nuclei generated in oocyte extract)
Specification Nuclei generated in oocyte extract
Fixative Formaldehyde
Permeabilization Yes - PBS-0.1% NP40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Andrew Lane

Verified customer

提交于 Apr 01 2013

Thank you for contacting us.
Both ab3742 and ab81371 have immunogen with 100% homology with the mouse sequence.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry and your interest in our products.
This particular antibody is not affinity purified but ascitic fluid so the concentration is not determined. As a general guidance, the concentration range is between: 5 - 10 mg/ml and t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (liver)
Specification liver
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37%FORMALDEHYDE
Detection step Semiquantitative PCR
Positive control pol 11
Negative control IgG
Username

Mr. Natarajan Balasubramaniyan

Verified customer

提交于 May 19 2012

Ab32058 DISCOUNT CODE: Expiration date: Ab32058 DISCOUNT CODE: Expiration date: Ab81371 DISCOUNT CODE: Expiration date: 14 May 2012 Ab75854 DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and testthes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"