Anti-Sumo 1抗体- Aminoterminal end (ab62517)

概述

  • 产品名称Anti-Sumo 1抗体- Aminoterminal end
    参阅全部 Sumo 1 一抗
  • 描述
    兔多克隆抗体to Sumo 1 - Aminoterminal end
  • 经测试应用适用于: ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide of 14 amino acids from near the N-terminus of human Sumo 1

  • 阳性对照
    • HL-60 cells

性能

应用

Our Abpromise guarantee covers the use of ab62517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC Use a concentration of 5 µg/ml.

靶标

  • 功能Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
  • 疾病相关Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
  • 序列相似性Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
  • 细胞定位Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAP1 antibody
    • GAP modifying protein 1 antibody
    • GAP-modifying protein 1 antibody
    • GMP 1 antibody
    • GMP1 antibody
    • OFC10 antibody
    • PIC 1 antibody
    • PIC1 antibody
    • SENP2 antibody
    • Sentrin 1 antibody
    • Sentrin antibody
    • Small ubiquitin related modifier 1 antibody
    • Small ubiquitin-like modifier 1 antibody
    • Small ubiquitin-related modifier 1 antibody
    • SMT3 antibody
    • SMT3 homolog 3 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3, yeast, homolog 3 antibody
    • Smt3C antibody
    • SMT3H3 antibody
    • SUMO-1 antibody
    • SUMO1 antibody
    • SUMO1_HUMAN antibody
    • Ubiquitin homology domain protein PIC1 antibody
    • Ubiquitin Like 1 antibody
    • Ubiquitin like protein SMT3C antibody
    • Ubiquitin like protein UBL1 antibody
    • Ubiquitin-homology domain protein PIC1 antibody
    • Ubiquitin-like protein SMT3C antibody
    • Ubiquitin-like protein UBL1 antibody
    • UBL 1 antibody
    • UBL1 antibody
    see all

Anti-Sumo 1 antibody - Aminoterminal end 图像

  • Lane 1 : Anti-Sumo 1 antibody - Aminoterminal end (ab62517) at 0.5 µg/ml
    Lane 2 : Anti-Sumo 1 antibody - Aminoterminal end (ab62517) at 1 µg/ml
    Lane 3 : Anti-Sumo 1 antibody - Aminoterminal end (ab62517) at 2 µg/ml

    Lane 1 : HL-60 cell lysate
    Lane 2 : HL-60 cell lysate
    Lane 3 : HL-60 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Immunocytochemistry of HL60 cells using ab62517 at 5 µg/ml.

Anti-Sumo 1 antibody - Aminoterminal end (ab62517)参考文献

ab62517 has not yet been referenced specifically in any publications.

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