概述

  • 产品名称Anti-Sumo 1抗体
    参阅全部 Sumo 1 一抗
  • 描述
    兔多克隆抗体to Sumo 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Arabidopsis thaliana
  • 免疫原

    Recombinant full length protein (Arabidopsis thaliana).

  • 阳性对照
    • Arabidopsis extracts that have been heat treated for for 30-120 mins by moving them from 24 to 37 degrees C. Recombinant SUMO1 is detectable at concentrations as low as 5ng/ml.

性能

应用

Our Abpromise guarantee covers the use of ab5316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 12 kDa.

靶标

  • 功能Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
  • 疾病相关Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
  • 序列相似性Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
  • 细胞定位Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • 别名
    • DAP1 antibody
    • GAP modifying protein 1 antibody
    • GAP-modifying protein 1 antibody
    • GMP 1 antibody
    • GMP1 antibody
    • OFC10 antibody
    • PIC 1 antibody
    • PIC1 antibody
    • SENP2 antibody
    • Sentrin 1 antibody
    • Sentrin antibody
    • Small ubiquitin related modifier 1 antibody
    • Small ubiquitin-like modifier 1 antibody
    • Small ubiquitin-related modifier 1 antibody
    • SMT3 antibody
    • SMT3 homolog 3 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3, yeast, homolog 3 antibody
    • Smt3C antibody
    • SMT3H3 antibody
    • SUMO-1 antibody
    • SUMO1 antibody
    • SUMO1_HUMAN antibody
    • Ubiquitin homology domain protein PIC1 antibody
    • Ubiquitin Like 1 antibody
    • Ubiquitin like protein SMT3C antibody
    • Ubiquitin like protein UBL1 antibody
    • Ubiquitin-homology domain protein PIC1 antibody
    • Ubiquitin-like protein SMT3C antibody
    • Ubiquitin-like protein UBL1 antibody
    • UBL 1 antibody
    • UBL1 antibody
    see all

Anti-Sumo 1 antibody 图像

  • Western blots of SUMO1 and SUMO3 antibody on arabidopsis extracts heat treated for the indicated times by moving them from 24 to 37 degrees C. The panel on the left was using ab5316, the panel on the right was using ab5317 - both antibodies were used at a dilution of 1/1000.

Anti-Sumo 1 antibody (ab5316)参考文献

This product has been referenced in:
  • Hermkes R  et al. Distinct roles for Arabidopsis SUMO protease ESD4 and its closest homolog ELS1. Planta 233:63-73 (2011). WB ; Arabidopsis thaliana . Read more (PubMed: 20922545) »
  • Budhiraja R  et al. Substrates related to chromatin and to RNA-dependent processes are modified by Arabidopsis SUMO isoforms that differ in a conserved residue with influence on desumoylation. Plant Physiol 149:1529-40 (2009). WB ; Arabidopsis thaliana . Read more (PubMed: 19151129) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Plants Tissue lysate - whole (anther)
Loading amount 30 µg
Specification anther
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 05 2012

Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with ab5316. The replacement vials are being sent to your attention free of charge on order reference # 109485. The item is currently out of stock and so you shoul...

Read More

Thank you for getting back to me and for providing further information about your experiments. As we understand from your recent e-mail, after blocking the membrane (without running the samples) the primary antibody provided a very high background s...

Read More

Thank you for contacting us and for taking your time to fill in the Questionnaire and for providing more information about your Western blot assay. This antibody was raised against a recombinant full length protein from Arabidopsis thaliana and it ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"