概述

  • 产品名称
    Anti-SULT2B1抗体
  • 描述
    兔多克隆抗体to SULT2B1
  • 经测试应用
    适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (

    YSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFI

    ) of Human SULT2B1 (NP_004596).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay is 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
  • 组织特异性
    Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
  • 序列相似性
    Belongs to the sulfotransferase 1 family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alcohol sulfotransferase antibody
    • EC 2.8.2.2 antibody
    • HSST2 antibody
    • Hydroxysteroid sulfotransferase 2 antibody
    • ST2B1 antibody
    • ST2B1_HUMAN antibody
    • Sulfotransferase 2B1 antibody
    • Sulfotransferase family cytosolic 2B member 1 antibody
    • SULT2B1 antibody
    • SULT2B1a antibody
    • SULT2B1b antibody
    see all

Anti-SULT2B1 antibody 图像

  • Anti-SULT2B1 antibody (ab82865) at 1 µg/ml (5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Anti-SULT2B1 antibody (ab82865) at 1/1000 dilution + whole cell lysate prepared from murine macrophages at 30 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-SULT2B1 antibody (ab82865)参考文献

This product has been referenced in:
  • Segala G  et al. 5,6-Epoxy-cholesterols contribute to the anticancer pharmacology of Tamoxifen in breast cancer cells. Biochem Pharmacol 86:175-89 (2013). Read more (PubMed: 23500540) »
  • Yang X  et al. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo. PLoS One 8:e60853 (2013). WB, ICC/IF ; Mouse, Human . Read more (PubMed: 23593328) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (liver cells)
Specification
liver cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages)
Loading amount
30 µg
Specification
macrophages
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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