概述

  • 产品名称
    Anti-SULT2A1抗体
    参阅全部 SULT2A1 一抗
  • 描述
    兔多克隆抗体to SULT2A1
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human SULT2A1 aa 253-285 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

性能

应用

Our Abpromise guarantee covers the use of ab38416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
  • 组织特异性
    Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
  • 序列相似性
    Belongs to the sulfotransferase 1 family.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

图片

  • Anti-SULT2A1 antibody (ab38416) at 1/100 dilution + Mouse kidney tissue lysate.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa


  • Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Both lanes: SULT2A1 antibody (ab38416) at 1/500 dilution (in PBS + 0.1% Tween20) for 1 hour at 22°C + whole tissue lysate of human liver at 20 µg.

    Secondary
    An HRP conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time: 1 minute 20 seconds

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

  • IHC image of ab38416 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38416, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of Anti-SULT2A1 antibody (ab38416) stained human ES cell derived hepatocytes. The cells were fixed in methanol, then blocked and permeabilized using BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab38416 at a 1/250 dilution for 16 hours at 4oC. The secondary antibody was a Goat anti-Rabbit Alexa Fluor 568, used at a 1/400 dilution.

    See Abreview

文献

This product has been referenced in:
  • Oostdijk W  et al. PAPSS2 deficiency causes androgen excess via impaired DHEA sulfation--in vitro and in vivo studies in a family harboring two novel PAPSS2 mutations. J Clin Endocrinol Metab 100:E672-80 (2015). Read more (PubMed: 25594860) »
  • Yalcin EB  et al. Downregulation of sulfotransferase expression and activity in diseased human livers. Drug Metab Dispos 41:1642-50 (2013). Read more (PubMed: 23775849) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Human Cell (ES - derived hepatocytes)
Specification
ES - derived hepatocytes
Permeabilization
Yes - During blocking step, BSA is diluted in PBS/0.1% Tween.
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (liver)
Loading amount
20 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Adreanl Carcinoma)
Specification
Adreanl Carcinoma
Fixative
Methanol
Permeabilization
Yes - 1% TX100
Username

Abcam user community

Verified customer

提交于 Oct 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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