概述

  • 产品名称
    Anti-Sulfatase 2抗体[2B4]
    参阅全部 Sulfatase 2 一抗
  • 描述
    小鼠单克隆抗体[2B4] to Sulfatase 2
  • 特异性
    ab113405 shows no cross-reactivity with Sulfatase 1
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant Human Sulfatase 2.

  • 表位
    ab113405 recognises an epitope located in the C terminal domain of Sulfatase 2.
  • 阳性对照
    • Sulfatase 2 transfected HEK29 cells or non-small-cell Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab113405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa. ab113405 detects a major band of approximately 50kDa in Sulfatase 2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.

靶标

  • 功能
    Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • 组织特异性
    Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
  • 序列相似性
    Belongs to the sulfatase family.
  • 翻译后修饰
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 细胞定位
    Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Extracellular sulfatase Sulf 2 antibody
    • Extracellular sulfatase Sulf-2 antibody
    • HSulf-2 antibody
    • HSulf2 antibody
    • Sulf2 antibody
    • SULF2_HUMAN antibody
    see all

图片

  • Paraffin embedded HEK293 cells transfected with Sulfatase 2 stained with ab113405.

文献

This product has been referenced in:
  • Lemjabbar-Alaoui H  et al. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene 29:635-46 (2010). Read more (PubMed: 19855436) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T Transfected with SULF2 over expressing pl)
Permeabilization
Yes - .1% Triton x-100 in 1XPBS with 1 % Goat Serum
Specification
HEK 293T Transfected with SULF2 over expressing pl
Blocking step
5% Goat serum in 1XPBS with 0.01% Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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提交于 Jul 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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