Anti-Substance P抗体[SP-DE4-21] (ab14184)

概述

  • 产品名称Anti-Substance P抗体[SP-DE4-21]
    参阅全部 Substance P 一抗
  • 描述
    小鼠单克隆抗体[SP-DE4-21] to Substance P
  • 特异性The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
  • 经测试应用适用于: ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
    预测可用于: Horse, Chicken, Hamster, Cow, Shark, Marmoset (common)
  • 免疫原

    Synthetic peptide corresponding to Human Substance P aa 58-68.
    Sequence:

    RPKPQQFFGLM


    Database link: P20366

  • 阳性对照
    • Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.

性能

应用

Our Abpromise guarantee covers the use of ab14184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/1000 - 1/2000.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-Substance P antibody [SP-DE4-21] 图像

  • ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

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  • Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
  • ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

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  • ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

  • ab14184 detecting Substance P in Human Serum samples by sandwich ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/400 in PBS) for 20 hours at 4°C. A biotinylated Rabbit anti-Substance P polyclonal (1/200) was used as the secondary antibody.

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Anti-Substance P antibody [SP-DE4-21] (ab14184)参考文献

This product has been referenced in:
  • Tratnjek L  et al. Upregulation and axonal transport of synaptotagmin-IV in the direct-pathway medium spiny neurons in hemi-parkinsonian rats induced by dopamine D1 receptor stimulation. Eur J Neurosci 43:885-98 (2016). Read more (PubMed: 26750488) »
  • Arosh JA  et al. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci U S A 112:9716-21 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26199416) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA and normal serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Rat Cell (Rat, free-floating brain slices, striatum and subs)
Specification Rat, free-floating brain slices, striatum and subs
Permeabilization Yes - Triton x-100
Fixative Paraformaldehyde
Username

Dr. Larisa Tratnjek

Verified customer

提交于 Mar 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Brain and jejunum)
Specification Brain and jejunum
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Pig Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Marmoset (common) Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 20 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cat Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Goat Tissue sections (Small intestine)
Specification Small intestine
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 18 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Dog Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Nov 18 2014

We have seen that enzymatic antigen retrieval is very successful when using this product on tissue in IHC-P and would recommend this. Have you attempted use of this type of antigen retrieval? I have added a link to Abcam's antigen retrieval guide for y...

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