The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
功能Probable pseudophosphatase. Contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Seems to play a role in spermiogenesis.
序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.
Protein tyrosine phosphatase like protein antibody
Protein tyrosine phosphatase-like protein antibody
Serine/threonine/tyrosine interacting protein antibody
Serine/threonine/tyrosine-interacting protein antibody
Anti-STYX antibody 图像
Western blot - STYX antibody (ab68016)
All lanes : Anti-STYX antibody (ab68016) at 1/500 dilution
Lane 1 : STYX transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa Observed band size : 25 kDa
Anti-STYX antibody (ab68016)参考文献
This product has been referenced in:
Reiterer V et al. Pseudophosphatase STYX modulates cell-fate decisions and cell migration by spatiotemporal regulation of ERK1/2. Proc Natl Acad Sci U S A110:E2934-43 (2013).
Read more (PubMed: 23847209) »