概述

  • 产品名称
  • 描述
    兔多克隆抗体to Styk1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 214-400 of Human Styk1 (NP_060893).

  • 阳性对照
    • A431 and MOLT4 whole cell lysates; Breast CA; 293T, H1299, HeLaS3 and Raji cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab97451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/250.

靶标

  • 功能
    Probable tyrosine protein-kinase, which has strong transforming capabilities on a variety of cell lines. When overexpressed, it can also induce tumor cell invasion as well as metastasis in distant organs. May act by activating both MAP kinase and phosphatidylinositol 3'-kinases (PI3K) pathways.
  • 组织特异性
    Widely expressed. Highly expressed in brain, placenta and prostate. Expressed in tumor cells such as hepatoma cells LO2, cervix carcinoma cells HeLa, ovary cancer cells Ho8910 and chronic myelogenous leukemia cells K562, but not in other tumor cells such as epidermoid carcinoma (A431). Undetectable in most normal lung tissues, widely expressed in lung cancers.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 protein kinase domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp761P1010 antibody
    • NOK antibody
    • Novel oncogene with kinase domain antibody
    • Protein kinase STYK1 antibody
    • Protein PK unique antibody
    • Protein PK-unique antibody
    • Serine/threonine/tyrosine kinase 1 antibody
    • Styk 1 antibody
    • STYK1 antibody
    • STYK1_HUMAN antibody
    • SuRTK 106 antibody
    • SuRTK106 antibody
    • Tyrosine protein kinase STYK1 antibody
    • Tyrosine-protein kinase STYK1 antibody
    see all

图片

  • All lanes : Anti-Styk1 antibody (ab97451) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
  • ab97451 at 1/100 dilution staining Styk1 in paraffin-embedded Breast CA by Immunohistochemistry.

文献

This product has been referenced in:
  • Chen MY  et al. Depletion of STYK1 inhibits intrahepatic cholangiocarcinoma development both in vitro and in vivo. Tumour Biol 37:14173-14181 (2016). Read more (PubMed: 27542675) »
  • Hu L  et al. Serine threonine tyrosine kinase 1 is a potential prognostic marker in colorectal cancer. BMC Cancer 15:246 (2015). IHC . Read more (PubMed: 25884558) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Loading amount
50 µg
Specification
293 cells
Treatment
mock tansfection vs. cells transfected with STYK1-FLAG construct
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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提交于 Jun 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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