重组Anti-STUB1/CHIP抗体[EPR4448] (ab109103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4448] to STUB1/CHIP
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-STUB1/CHIP抗体[EPR4448]
参阅全部 STUB1/CHIP 一抗 -
描述
兔单克隆抗体[EPR4448] to STUB1/CHIP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, MCF7, ECV304, and 293T cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4448 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
|
ICC/IF |
1/100 - 1/250.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
组织特异性
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 3 TPR repeats.
Contains 1 U-box domain. -
结构域
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10273 Human
- Entrez Gene: 56424 Mouse
- Entrez Gene: 287155 Rat
- Omim: 607207 Human
- SwissProt: Q9UNE7 Human
- SwissProt: Q9WUD1 Mouse
- Unigene: 592081 Human
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别名
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
图片
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All lanes : Anti-STUB1/CHIP antibody [EPR4448] (ab109103) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : ECV304 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
Immunofluorescent staining of STUB1/CHIP in HeLa cells using ab109103 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109103 被引用在 4 文献中.
- Johnson OT et al. Two distinct classes of cochaperones compete for the EEVD motif in heat shock protein 70 to tune its chaperone activities. J Biol Chem 298:101697 (2022). PubMed: 35148989
- Sugatani J et al. Threonine-408 Regulates the Stability of Human Pregnane X Receptor through Its Phosphorylation and the CHIP/Chaperone-Autophagy Pathway. Drug Metab Dispos 44:137-50 (2016). PubMed: 26534988
- El-Kasaby A et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem 289:28987-9000 (2014). PubMed: 25202009
- Bergmayr C et al. Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. J Biol Chem 288:28831-44 (2013). PubMed: 23965991