概述

  • 产品名称
  • 描述
    山羊多克隆抗体to STUB1
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee
  • 免疫原

    Synthetic peptide:

    DAFISENGWVEDY

    , corresponding to C terminal amino acids 291-303 of Human STUB1

性能

应用

Our Abpromise guarantee covers the use of ab2482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    Peptide ELISA: Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 35 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
    • 组织特异性
      Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
    • 通路
      Protein modification; protein ubiquitination.
    • 序列相似性
      Contains 3 TPR repeats.
      Contains 1 U-box domain.
    • 结构域
      The TPR domain is essential for ubiquitination mediated by UBE2D1.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen NY CO 7 antibody
      • Antigen NY-CO-7 antibody
      • C terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70 interacting protein antibody
      • Carboxy terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70p interacting protein antibody
      • CHIP antibody
      • CHIP_HUMAN antibody
      • CLL associated antigen KW 8 antibody
      • CLL-associated antigen KW-8 antibody
      • E3 ubiquitin protein ligase CHIP antibody
      • E3 ubiquitin-protein ligase CHIP antibody
      • Heat shock protein A binding protein 2 (c terminal) antibody
      • HSPABP2 antibody
      • NY CO 7 antibody
      • PP1131 antibody
      • SDCCAG7 antibody
      • Serologically defined colon cancer antigen 7 antibody
      • STIP1 homology and U Box containing protein 1 antibody
      • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
      • STIP1 homology and U box-containing protein 1 antibody
      • STUB 1 antibody
      • STUB1 antibody
      • UBOX 1 antibody
      • UBOX1 antibody
      see all

    Anti-STUB1 antibody 图像

    • Anti-STUB1 antibody (ab2482) at 1 µg/ml + Human Heart lysate (RIPA buffer, 35ug total protein per lane).

      Predicted band size : 35 kDa

    实验方案

    Anti-STUB1 antibody (ab2482)参考文献

    This product has been referenced in:
    • Bento CF  et al. Methylglyoxal alters the function and stability of critical components of the protein quality control. PLoS One 5:e13007 (2010). WB ; Human . Read more (PubMed: 20885985) »
    • Bento CF  et al. The chaperone-dependent ubiquitin ligase CHIP targets HIF-1a for degradation in the presence of methylglyoxal. PLoS One 5:e15062 (2010). WB ; Human . Read more (PubMed: 21124777) »

    See all 2 Publications for this product

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