Anti-Strumpellin抗体(ab101222)
Key features and details
- Rabbit polyclonal to Strumpellin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Strumpellin抗体
参阅全部 Strumpellin 一抗 -
描述
兔多克隆抗体to Strumpellin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Zebrafish, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody can a positive signal in the following lysates: Mouse Brain Tissue; Mouse Spinal Cord Tissue; Mouse Lung Tissue; HeLa Whole Cell; HepG2 Whole Cell.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
靶标
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功能
Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. -
组织特异性
Expressed ubiquitously. -
疾病相关
Defects in KIAA0196 are the cause of spastic paraplegia autosomal dominant type 8 (SPG8) [MIM:603563]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
序列相似性
Belongs to the strumpellin family. - Information by UniProt
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数据库链接
- Entrez Gene: 9897 Human
- Entrez Gene: 223593 Mouse
- Entrez Gene: 100173723 Orangutan
- Entrez Gene: 393152 Zebrafish
- Omim: 610657 Human
- SwissProt: Q12768 Human
- SwissProt: Q8C2E7 Mouse
- SwissProt: Q5R5P0 Orangutan
see all -
别名
- KIAA0196 antibody
- SPG8 antibody
- STRUM_HUMAN antibody
- WASH complex subunit strumpellin antibody
图片
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ab101222 staining Strumpellin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab101222 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Strumpellin antibody (ab101222) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 45 kDa, 50 kDa, 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab101222 被引用在 4 文献中.
- Farina F et al. Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis. EMBO J 38:N/A (2019). PubMed: 31015335
- Ripoll L et al. Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers. J Cell Biol 217:2709-2726 (2018). PubMed: 29875258
- Tyrrell BJ et al. Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell Melanoma Res 29:559-71 (2016). PubMed: 27390154
- Wang F et al. WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes. Sci Rep 4:5596 (2014). Mouse . PubMed: 24998208