重组
RabMAb

Anti-Stromal interaction molecule 1抗体[EPR3414] (ab108994)

概述

  • 产品名称
    Anti-Stromal interaction molecule 1抗体[EPR3414]
    参阅全部 Stromal interaction molecule 1 一抗
  • 描述
    兔单克隆抗体[EPR3414] to Stromal interaction molecule 1
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Stromal interaction molecule 1.

  • 阳性对照
    • SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98 &degC, below boiling, and then let cool for 10-20 min.

  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
    • 组织特异性
      Ubiquitously expressed in various human primary cells and tumor cell lines.
    • 疾病相关
      Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
    • 序列相似性
      Contains 1 EF-hand domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • 结构域
      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • 翻译后修饰
      Glycosylation is required for cell surface expression.
      Phosphorylated predominantly on Ser residues.
    • 细胞定位
      Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • D11S4896E antibody
      • GOK antibody
      • OTTHUMP00000164512 antibody
      • OTTHUMP00000229140 antibody
      • OTTHUMP00000230742 antibody
      • SIM antibody
      • STIM 1 antibody
      • STIM1 antibody
      • Stim1 stromal interaction molecule 1 antibody
      • STIM1_HUMAN antibody
      • STIM1L antibody
      • Stromal interaction molecule 1 antibody
      see all

    图片

    • All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

      Lane 1 : SH SY5Y cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.


      Performed under reducing conditions.

      Predicted band size : 77 kDa
    • ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

    文献

    This product has been referenced in:
    • Wu G  et al. Overexpression of stromal interaction molecule 1 may promote epithelial-mesenchymal transition and indicate poor prognosis in gastric cancer. Mol Med Rep 16:151-158 (2017). IHC-P ; Human . Read more (PubMed: 28534934) »
    • Han S  et al. Anti-allergic activity of glycyrrhizic acid on IgE-mediated allergic reaction by regulation of allergy-related immune cells. Sci Rep 7:7222 (2017). Read more (PubMed: 28775294) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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