The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Streptococcus pneumoniae are Gram positive diplococci, alpha hemolytic aerotolerant anaerobes. S. pneumoniae is a significant human pathogen, being recognized as a major cause of pneumonia in the late 19th century, and is currently the subject of many humoral immunity studies.
S. pneumoniae is a transient member of the normal flora, colonizing the nasopharynx of 40% of healthy adults and children with no adverse effects. There are more than 90 serotypes: serotypes 6, 14, 18, 19, and 23 are the most prevalent accounting for 60-80% of infections depending on the area of the world. Pneumococcal infection accounts for more deaths than any other vaccine preventable bacterial disease.
S. pneumoniae can express several different virulence factors which contribute to some of the clinical manifestations seen during infection.