Type specific carbohydrate for group A Streptococcus. No immunological cross reactivity. However, there is potential non-specific reactivity with such organisms as group C Streptococcus and Staphyloccus due to the presence of protein A like proteins (Staphyloccus) and protein C like proteins (Streptococcus C) present on the intact surface of these bacteria. These proteins bind any immunoglobulin (including the FITC Conjugate) and therefore will appear to fluoresce (non-specifically
Reacts with Streptococcus group A.
Not yet tested in other species.
Tissue/ cell preparation.Streptococci, Group A
Immunoaffinity purified antibody covalently coupled with high purity Isomer I of fluorescein isothiocyanate (F:P ratio=5.4). Care is taken to ensure complete removal of any free fluorescein from the final product.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/10 - 1/50.
IF: 1/10 - 1/50.
Direct FA staining of target antigens in a permissive tissue culture system. Functions both as capture and detection antibody. Acetone fixation of the antigen source is recommended prior to staining. Pre-block slide with 10% normal goat serum.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nares and pharynx. However, in certain circumstances, they may also inhabit the skin, heart or muscle tissue.