Streptavidin Peroxidase (Ready to Use) (ab64269)


  • 产品名称
    Streptavidin Peroxidase (Ready to Use)
  • 经测试应用
    适用于: IHC-P, Sandwich ELISAmore details
  • 常规说明

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Add enough drops of an appropriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.

    3. Perform appropriate pretreatment if required. Wash 3 times in buffer.

    4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.

    5. Apply primary antibody and incubate according to manufacturer's protocol.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary solution according to manufacturer’s protocol. Wash 4 times in buffer.

    7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.

    9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.


  • 相关性
    Streptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.


Our Abpromise guarantee covers the use of ab64269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. PubMed: 21371998

Streptavidin Peroxidase (Ready to Use) 图像

  • Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).

Streptavidin Peroxidase (Ready to Use) (ab64269)参考文献

This product has been referenced in:
  • LaPlante CD & Vandenberg LN Data describing lack of effects of 17a-ethinyl estradiol on mammary gland morphology in female mice exposed during pregnancy and lactation. Data Brief 14:337-343 (2017). Read more (PubMed: 28795111) »
  • Wilson R  et al. Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLoS One 9:e100572 (2014). Read more (PubMed: 24963654) »

See all 3 Publications for this product

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Les deux kits que je vous recommende sont :

Streptavidin Alkaline Phosphatase (Ready to Use),
Streptavidin Peroxidase (Ready to Use),


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