Anti-Streptavidin抗体[S10D4] (ab10020)

概述

  • 产品名称Anti-Streptavidin抗体[S10D4]
    参阅全部 Streptavidin 一抗
  • 描述
    小鼠单克隆抗体[S10D4] to Streptavidin
  • 特异性Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
  • 经测试应用适用于: ICC, WB, IHC-P, IHC-Fr, ICC/IF, Other, ELISA, Flow Cytmore details
  • 免疫原

    Streptavidin.

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purity tested by elcetrophoresis.
  • 克隆单克隆
  • 克隆编号S10D4
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
AP Use at an assay dependent concentration.

靶标

  • 相关性Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • 细胞定位Cytoplasmic
  • 别名
    • SA V1 antibody
    • SA V2 antibody
    • strepavidin antibody
    • Streptavidin V1 antibody
    • Streptavidin V2 antibody
    see all

Anti-Streptavidin antibody [S10D4] 图像

  • Immunofluorescence analysis of Potorous tridactylis kidney (PtK2) cells, staining Streptavidin with ab10020.

    Cells were fixed with 4% paraformaldehyde, before being incubated with wt streptavidin (2 µM) for 5 min at room temperature. Samples were incubated with 1 µL primary antibody for 1 hour at 4°C. A FITC-conjugated goat anti-mouse IgG was used as the secondary antibody.

Anti-Streptavidin antibody [S10D4] (ab10020)参考文献

This product has been referenced in:
  • Bertuccio CA  et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9:e92013 (2014). Read more (PubMed: 24632741) »
  • Dykstra T  et al. Defined particle ligands trigger specific defense mechanisms of macrophages. Innate Immun 17:388-402 (2011). Read more (PubMed: 20682584) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8.5% gel)
Sample Human Cell lysate - whole cell (HEK 293)
Specification HEK 293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2014

Normally 10-50 ug of lysate protein is required for an antibody detection in western blot, however if you want to detect amount lower than this then you may need to isolate the protein or purify the protein form the cell lysates. e.g. antibody can easi...

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Vielen Dank für Ihre Anfrage.

Die Konzentration dieses Antikörpers ist davon abhängig, wie viel Streptavidin in Ihrem System eingesetzt wird.


Ein Kunde hat laut einer Rezession diesen Antikörper in einer 1/500...

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Thank you for contacting Abcam.

I think any of the any of the antibodies you are interested in would work for you in your desired applications. As they are mouse monoclonals, you may see some mouse on mouse staining and unfortunately we do no...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich genauer in den Laboren nachgefragt, die unsere Streptavidin-Antikörper herstellen, und dabei hat sich herausgestellt, dass unsere monoklonalen anti-Streptavidin-Antikörper (ab10020-10023)...

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Thank your for your inquiry.

I can confirm that ab59320 is now unpublished and not available any more. I am sorry if this causes any inconvenience.

ab6676 is a polyclonal antibody that has the whole streptavidin molecule as immuno...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (hippocampal neurons)
Specification hippocampal neurons
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2008

Thank you for your enquiry. The affinity constant of ab10020 has been determined to be >1x10E8. Please do not hesitate to contact us if you have additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"