Anti-Streptavidin抗体[S10D4] (ab10020)

概述

  • 产品名称
    Anti-Streptavidin抗体[S10D4]
    参阅全部 Streptavidin 一抗
  • 描述
    小鼠单克隆抗体[S10D4] to Streptavidin
  • 特异性
    Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
  • 经测试应用
    适用于: ICC, WB, IHC-P, IHC-Fr, ICC/IF, Other, ELISA, Flow Cytmore details
  • 免疫原

    Streptavidin.

  • 常规说明
    Concentration varies from lot to lot and can be provided on request.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purity tested by elcetrophoresis.
  • 克隆
    单克隆
  • 克隆编号
    S10D4
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
AP Use at an assay dependent concentration.

靶标

  • 相关性
    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • 细胞定位
    Cytoplasmic
  • 别名
    • SA V1 antibody
    • SA V2 antibody
    • strepavidin antibody
    • Streptavidin V1 antibody
    • Streptavidin V2 antibody
    see all

Anti-Streptavidin antibody [S10D4] 图像

  • Immunofluorescence analysis of Potorous tridactylis kidney (PtK2) cells, staining Streptavidin with ab10020.

    Cells were fixed with 4% paraformaldehyde, before being incubated with wt streptavidin (2 µM) for 5 min at room temperature. Samples were incubated with 1 µL primary antibody for 1 hour at 4°C. A FITC-conjugated goat anti-mouse IgG was used as the secondary antibody.

Anti-Streptavidin antibody [S10D4] (ab10020)参考文献

This product has been referenced in:
  • Bertuccio CA  et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9:e92013 (2014). Read more (PubMed: 24632741) »
  • Dykstra T  et al. Defined particle ligands trigger specific defense mechanisms of macrophages. Innate Immun 17:388-402 (2011). Read more (PubMed: 20682584) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8.5% gel)
Sample
Human Cell lysate - whole cell (HEK 293)
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2014

Normally 10-50 ug of lysate protein is required for an antibody detection in western blot, however if you want to detect amount lower than this then you may need to isolate the protein or purify the protein form the cell lysates. e.g. antibody can easi...

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Vielen Dank für Ihre Anfrage.

Die Konzentration dieses Antikörpers ist davon abhängig, wie viel Streptavidin in Ihrem System eingesetzt wird.


Ein Kunde hat laut einer Rezession diesen Antikörper in einer 1/500...

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Thank you for contacting Abcam.

I think any of the any of the antibodies you are interested in would work for you in your desired applications. As they are mouse monoclonals, you may see some mouse on mouse staining and unfortunately we do no...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich genauer in den Laboren nachgefragt, die unsere Streptavidin-Antikörper herstellen, und dabei hat sich herausgestellt, dass unsere monoklonalen anti-Streptavidin-Antikörper (ab10020-10023)...

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Thank your for your inquiry.

I can confirm that ab59320 is now unpublished and not available any more. I am sorry if this causes any inconvenience.

ab6676 is a polyclonal antibody that has the whole streptavidin molecule as immuno...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (hippocampal neurons)
Specification
hippocampal neurons
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2008

Thank you for your enquiry. The affinity constant of ab10020 has been determined to be >1x10E8. Please do not hesitate to contact us if you have additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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