Anti-Stra8抗体(ab49602)
Key features and details
- Rabbit polyclonal to Stra8
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Stra8抗体
参阅全部 Stra8 一抗 -
描述
兔多克隆抗体to Stra8 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Hamster -
免疫原
Synthetic peptide corresponding to Mouse Stra8 aa 1-100.
(Peptide available asab49601) -
阳性对照
- IHC-P: Mouse testis tissue. WB: CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49602于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (3) |
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.
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说明 |
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IHC-P
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. |
靶标
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功能
Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. -
组织特异性
Expressed specifically in testis. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 20899 Mouse
- Entrez Gene: 500079 Rat
- SwissProt: P70278 Mouse
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别名
- Stimulated by retinoic acid 8 antibody
- stimulated by retinoic acid 8 homolog antibody
- Stimulated by retinoic acid Gene 8 antibody
see all
图片
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IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-Stra8 antibody (ab49602) at 1 µg/ml
Lane 1 : CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate
Lane 2 : CHO Negative Control Whole Cell Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. -
All lanes : Anti-Stra8 antibody (ab49602) at 1/500 dilution
Lane 1 : WT D3 mouse ES cells cultured with LIF.
Lane 2 : WT D3 mouse ES cells cultured without LIF for 24 hours.
Lane 3 : WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours.
Lysates/proteins at 200000 cells per lane.
Secondary
All lanes : Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (76)
ab49602 被引用在 76 文献中.
- Cao Y et al. Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs. J Vet Sci 23:e35 (2022). PubMed: 35363442
- Liu X et al. Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide. J Cell Mol Med 25:1624-1632 (2021). PubMed: 33438283
- Xu Y & Xie J Etomoxir regulates the differentiation of male germ cells by specifically reducing H3K27ac level. BMC Dev Biol 21:5 (2021). PubMed: 33517883
- Zhang M et al. Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling. Stem Cells Int 2021:6668658 (2021). PubMed: 33603791
- Ikeda Y et al. The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation. Sci Rep 11:4486 (2021). PubMed: 33627800