概述

  • 产品名称Anti-Stra8抗体
    参阅全部 Stra8 一抗
  • 描述
    兔多克隆抗体to Stra8
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Stra8.

    (Peptide available as ab49600.)

  • 阳性对照
    • This antibody gave a positive signal in WB in Mouse brain tissue lysate and Stra8 (Mouse) Transient Overexpression Lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab49405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/250.

靶标

  • 功能Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase.
  • 组织特异性Expressed specifically in testis.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • stimulated by retinoic acid 8 homolog antibody
    • Stimulated by retinoic acid Gene 8 antibody
    • stimulated by retinoic acid gene 8 homolog antibody
    • Stimulated by retinoic acid gene 8 protein homolog antibody
    • STRA 8 antibody
    • STRA8 antibody
    • STRA8_HUMAN antibody
    see all

Anti-Stra8 antibody 图像

  • Anti-Stra8 antibody (ab49405) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 55 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Stra8 antibody (ab49405) at 1 µg/ml

    Lane 1 : CHO overexpressing Mouse Stra8 lysate
    Lane 2 : CHO Negative Control Lysate

    Lysates/proteins at 1 µl per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Ab49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.

  • IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Stra8 antibody (ab49405)参考文献

This product has been referenced in:
  • Miles DC  et al. Signaling through the TGF beta-activin receptors ALK4/5/7 regulates testis formation and male germ cell development. PLoS One 8:e54606 (2013). ICC/IF ; Mouse . Read more (PubMed: 23342175) »
  • Sleutels F  et al. The male germ cell gene regulator CTCFL is functionally different from CTCF and binds CTCF-like consensus sites in a nucleosome composition-dependent manner. Epigenetics Chromatin 5:8 (2012). IHC-P ; Mouse . Read more (PubMed: 22709888) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Loading amount 200000 cells
Specification mouse embryonic stem cell
Treatment 1uM RA for 24 hours
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

Merci de nous avoir contactés.

Comme convenu vendredi, j'ai mis en place l'envoi d'une unité de ab49602 en remplacement du ab49405 qui n'a pas fonctionné. Le numéro de commande de remplacement gratuit de ce produit ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Feb 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"