概述

  • 产品名称
    Anti-STON2抗体
  • 描述
    兔多克隆抗体to STON2
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 299-437 of Human STON2.

  • 阳性对照
    • Human fallopian tube tissue; A431 cells.

性能

应用

Our Abpromise guarantee covers the use of ab121165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 fixation.
WB 1/250 - 1/500. Predicted molecular weight: 101 kDa.

靶标

  • 功能
    Adapter protein involved in endocytic machinery. May be involved in the vesicle recycling. May facilitate clathrin-coated vesicle uncoating.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the Stoned B family.
    Contains 1 MHD (mu homology) domain.
    Contains 1 SHD (stonin homology) domain.
  • 结构域
    The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of SYT1, SYT2, EPS15, EPS15R and ITSN1.
  • 细胞定位
    Cytoplasm. Membrane. Some fraction is membrane-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4933401N24Rik antibody
    • AI425999 antibody
    • AW544599 antibody
    • Homolog of stoned B antibody
    • RGD1311502 antibody
    • STN2 antibody
    • STNB antibody
    • STNB2 antibody
    • STON2 antibody
    • STON2_HUMAN antibody
    • Stoned B antibody
    • Stonin-2 antibody
    see all

Anti-STON2 antibody 图像

  • ab121165 at 1/125 dilution staining STON2 in Paraffin-emebedded Human fallopian tube tissue by Immunohistochemistry.

    Note: moderate cytoplasmic positivity in glandular cells.
  • ab121165 at 4 µg/ml staining STON2 in PFA/Triton X-100 fixed A431 cells by Immunofluorescence(green).

    Note: positivity in nucleoli and cytoplasm.
  • Anti-STON2 antibody (ab121165) + Human cell line K562

    Predicted band size : 101 kDa

Anti-STON2 antibody (ab121165)参考文献

ab121165 has not yet been referenced specifically in any publications.

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