The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
Use at an assay dependent concentration.
1/200. (see Abreview)
ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
功能Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
序列相似性Belongs to the band 7/mec-2 family.
细胞定位Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunocytochemistry/ Immunofluorescence - STOM antibody (ab67880)This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa
Central Panel: ab67880 staining STOM in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/250, was used as secondary antibody. Left-hand panel: Nuclei counterstained with DAPI (blue). Right-hand panel: Overlay