STO全细胞裂解液(ab3874)

概述

  • 产品名称STO全细胞裂解液
  • 常规说明Source - Mouse embryonic fibroblasts.


    STO whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 5% Beta mercaptoethanol
    Note: 1xSDS buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 裂解物说明STO whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域
  • 背景These immortal cells are used as a monolayer of feeder cells to embryonic stem cells (in particular in human stem cell research). Cells are SIM mouse embryo-derived and thioguanine and ouabain resistant.

应用

Our Abpromise guarantee covers the use of ab3874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. STO whole cell lysate is ready to load on SDS-PAGE for Western blotting. We recommend loading 20 µg per lane for mini gels.

STO whole cell lysate (ab3874)参考文献

ab3874 has not yet been referenced specifically in any publications.

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Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
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I'm sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell ...

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