概述

  • 产品名称
  • 描述
    山羊多克隆抗体to STMN2
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide:

    AKTAMAYKEK

    , corresponding to N terminal amino acids 2-11 of STMN2

  • 阳性对照
    • Human Brain lysate, A431 lysate ICC/IF: HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa.

靶标

  • 功能
    Is a key regulator of neurite extension through regulation of microtubule instabilily.
  • 组织特异性
    Neuron specific.
  • 序列相似性
    Belongs to the stathmin family.
  • 翻译后修饰
    Sumoylated.
  • 细胞定位
    Cytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Neuron specific growth associated protein antibody
    • Neuronal growth associated protein antibody
    • Neuronal growth associated protein (silencer element) antibody
    • Protein SCG10 antibody
    • SCG 10 antibody
    • SCG10 antibody
    • SCG10 protein antibody
    • SCGN 10 antibody
    • SCGN10 antibody
    • SGC 10 antibody
    • SGC10 antibody
    • Stathmin 2 antibody
    • Stathmin like 2 antibody
    • Stathmin-2 antibody
    • STMN 2 antibody
    • STMN2 antibody
    • STMN2_HUMAN antibody
    • Superior cervical ganglia neural specific 10 antibody
    • Superior cervical ganglion 10 protein antibody
    • Superior cervical ganglion-10 protein antibody
    • Superiorcervical ganglia neural specific 10 antibody
    see all

图片

  • Ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-STMN2 antibody (ab21190) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 20.8 kDa
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

文献

This product has been referenced in:
  • Davis EJ  et al. Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion. Neuroscience 146:784-91 (2007). Read more (PubMed: 17331653) »
  • Ohkawa N  et al. The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. J Cell Sci 120:1447-56 (2007). Read more (PubMed: 17389683) »

See all 2 Publications for this product

客户评价及客户问答

I'm sorry to hear you are having a problem with ab21190. This antibody has been tested in human brain lysate lysed in RIPA buffer containing protease inhibitors. You do not mention which type of cell lysate you have used and it may be that your sam...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am most surprised by the results that your customer has been obtaining. We recommend that the antibody can be applied in a dilution range of ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab21190. Please note that this antibody is a "Fast-track" antibody and has not yet been fully characterized. Preliminary Western blot experiments were done using...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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