• 产品名称Anti-STK33抗体
    参阅全部 STK33 一抗
  • 描述
    小鼠单克隆抗体to STK33
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-515 of Human STK33


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab57693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • 组织特异性Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hypothetical protein FLJ35932 antibody
    • Serine threonine kinase 33 antibody
    • Serine/threonine kinase 33 antibody
    • Serine/threonine protein kinase 33 antibody
    • Serine/threonine-protein kinase 33 antibody
    • STK 33 antibody
    • Stk33 antibody
    • STK33_HUMAN antibody
    see all

Anti-STK33 antibody 图像

  • Predicted band size : 58 kDa
    STK33 antibody (ab57693) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57693 (4µg/ml) staining STK33 in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing A549 cells stained with ab57693 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57693, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min) /permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-STK33 antibody (ab57693)参考文献

ab57693 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 20 µg
Specification HeLa Cells
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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提交于 Jun 08 2012