Anti-STK31抗体- N-terminal (ab71698)

概述

  • 产品名称
    Anti-STK31抗体- N-terminal
    参阅全部 STK31 一抗
  • 描述
    兔多克隆抗体to STK31 - N-terminal
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide from the N terminal region of human STK31 conjugated to KLH.

  • 阳性对照
    • Rat testis tissue lysate. Human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab71698 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 116 kDa).

靶标

  • 组织特异性
    Testis specific.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Testis tissue sperm-binding protein Li 78m antibody
    • Tudor domain containing 8 antibody
    see all

图片

  • Anti-STK31 antibody - N-terminal (ab71698) at 1/100 dilution + rat testis tissue lysate at 12.5 µg

    Predicted band size : 116 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab71698, at 1/50 dilution, staining STK31 in human breast carcinoma by Immunohistochemistry using formalin fixed, paraffin embedded tissue. ab71698 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

文献

ab71698 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Loading amount
20 µg
Specification
ES cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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提交于 Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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