Anti-STK31抗体(ab76839)

概述

  • 产品名称Anti-STK31抗体
    参阅全部 STK31 一抗
  • 描述
    小鼠单克隆抗体to STK31
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Recombinant fragment with a tag: WLSVQNQEFE INKDGIPKVD QFHLDDKVKS LLCSLICYRS SMTAEQVLNA ECFLMPKEQS VPNPEKDTEY TLYKKEEEIK TENLDKCMEK TRNGEANFDC, corresponding to amino acids 920-1019 of human STK31 (NP_113602). The tag sequence is not included here.

  • 阳性对照
    • STK31 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 116 kDa.

靶标

  • 组织特异性Testis specific.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Tudor domain containing 8 antibody
    see all

Anti-STK31 antibody 图像

  • Anti-STK31 antibody (ab76839) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 116 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-STK31 antibody (ab76839) at 5 µg/ml

    Lane 1 : STK31 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 116 kDa
    Observed band size : 116 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-STK31 antibody (ab76839)参考文献

ab76839 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (ES cells)
Loading amount 20 µg
Specification ES cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"