Anti-STK3抗体[EP1466Y] (ab52641)
- 数据表
- 具体的参考文献 (14)
- 实验方案
概述
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产品名称Anti-STK3抗体[EP1466Y]
参阅全部 STK3 一抗 -
描述兔单克隆抗体[EP1466Y] to STK3
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宿主Rabbit
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经测试应用适用于: ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human STK3 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- ICC/IF: Wildtype HAP1 cells. WB: C6 cell lysate. IHC-P: Human lymphoma tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EP1466Y
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab52641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24595170 | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). | |
| IP | 1/50 - 1/100. | |
| IHC-P | 1/50 - 1/100. Antigen retrieval step strongly recommended for enhanced signal. |
靶标
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功能Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
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组织特异性Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
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序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
细胞定位Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 6788 Human
- Entrez Gene: 56274 Mouse
- Entrez Gene: 65189 Rat
- Omim: 605030 Human
- SwissProt: Q13188 Human
- SwissProt: Q9JI10 Mouse
- SwissProt: O54748 Rat
- Unigene: 492333 Human
see all -
别名
- 0610042I06Rik antibody
- EC 2.7.11.1 antibody
- FLJ90748 antibody
see all
图片
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![Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-254427-ab52641WTKO.jpg)
Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: STK3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: 293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52641 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.ab52641 was shown to specifically react with STK3 when STK3 knockout samples were used. Wild-type and STK3 knockout samples were subjected to SDS-PAGE. ab52641 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-269921-ICCGR273441501.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-STK3 antibody [EP1466Y] (ab52641)ab52641 staining STK3 in wild-type HAP1 cells (top panel) and STK3 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52641 at 5μg/ml concentration and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-4-ab52641IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 antibody [EP1466Y] (ab52641)Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab52641 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunoprecipitation - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-5-ab52641IP1.jpg)
Immunoprecipitation - Anti-STK3 antibody [EP1466Y] (ab52641)ab52641 (purified) at 1/50 immunoprecipitating STK3 in 10 μg C6 cell lysate (Lanes 1 and 2, observed at 56 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
![Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-257457-ab52641WB2.jpg)
Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)All lanes : Anti-STK3 antibody [EP1466Y] (ab52641) at 1/50000 dilution (purified)
Lane 1 : C6 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)](http://www.abcam.cn/ps/products/52/ab52641/Images/ab52641-257456-ab52641WB1.jpg)
Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)All lanes : Anti-STK3 antibody [EP1466Y] (ab52641) at 1/50000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
文献
This product has been referenced in:
- Papaspyropoulos A et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73. Nat Commun 9:424 (2018). Read more (PubMed: 29382819) »
- Girling KD et al. Activation of caspase-6 and cleavage of caspase-6 substrates is an early event in NMDA receptor-mediated excitotoxicity. J Neurosci Res 96:391-406 (2018). Read more (PubMed: 29193273) »
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