Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STK3 aa 1-100 (N terminal).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab52641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | Use a concentration of 5 µg/ml. | |
IHC-Fr | Use at an assay dependent concentration. PubMed: 24595170 | |
WB | 1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). | |
IP | 1/50 - 1/100. | |
IHC-P | 1/50 - 1/100. Antigen retrieval step strongly recommended for enhanced signal. |
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: STK3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: 293T cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52641 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52641 was shown to specifically react with STK3 when STK3 knockout samples were used. Wild-type and STK3 knockout samples were subjected to SDS-PAGE. ab52641 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab52641 staining STK3 in wild-type HAP1 cells (top panel) and STK3 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52641 at 5μg/ml concentration and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"