概述

  • 产品名称
    Anti-STK3抗体[EP1466Y]
    参阅全部 STK3 一抗
  • 描述
    兔单克隆抗体[EP1466Y] to STK3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STK3 aa 1-100 (N terminal).

  • 阳性对照
    • ICC/IF: Wildtype HAP1 cells. WB: C6 cell lysate. IHC-P: Human lymphoma tissue.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab52641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 24595170
WB 1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP 1/50 - 1/100.
IHC-P 1/50 - 1/100.

Antigen retrieval step strongly recommended for enhanced signal.
  • 应用说明
    Is unsuitable for Flow Cyt.

    • 靶标

    • 功能
      Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
    • 组织特异性
      Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
    • 序列相似性
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SARAH domain.
    • 细胞定位
      Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • 0610042I06Rik antibody
      • EC 2.7.11.1 antibody
      • FLJ90748 antibody
      • KB 1458E12.1 antibody
      • Kinase responsive to stress 1 antibody
      • KRS1 antibody
      • Mammalian STE20 like protein kinase 2 antibody
      • Mammalian STE20-like protein kinase 2 antibody
      • Mammalian sterile 20-like 2 antibody
      • Mess1 antibody
      • MST antibody
      • MST-2 antibody
      • MST2 antibody
      • Mst3 antibody
      • Serine/threonine kinase 3 (STE20 homolog, yeast) antibody
      • Serine/threonine kinase 3 (Ste20, yeast homolog) antibody
      • Serine/threonine kinase 3 antibody
      • Serine/threonine protein kinase 3 antibody
      • Serine/threonine protein kinase Krs1 antibody
      • Serine/threonine-protein kinase 3 antibody
      • Serine/threonine-protein kinase Krs-1 antibody
      • STE20 like kinase MST2 antibody
      • STE20-like kinase MST2 antibody
      • Stk3 antibody
      • STK3_HUMAN antibody
      • wu:fc19e11 antibody
      • zgc:55383 antibody
      see all

    图片

    • Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)
      Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: STK3 knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH/3T3 cell lysate (20 µg)
      Lane 4: 293T cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab52641 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab52641 was shown to specifically react with STK3 when STK3 knockout samples were used. Wild-type and STK3 knockout samples were subjected to SDS-PAGE. ab52641 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-STK3 antibody [EP1466Y] (ab52641)
      Immunocytochemistry/ Immunofluorescence - Anti-STK3 antibody [EP1466Y] (ab52641)

      ab52641 staining STK3 in wild-type HAP1 cells (top panel) and STK3 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52641 at 5μg/ml concentration and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 antibody [EP1466Y] (ab52641)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 antibody [EP1466Y] (ab52641)
      Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab52641 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunoprecipitation - Anti-STK3 antibody [EP1466Y] (ab52641)
      Immunoprecipitation - Anti-STK3 antibody [EP1466Y] (ab52641)
      ab52641 (purified) at 1/50 immunoprecipitating STK3 in 10 μg C6 cell lysate (Lanes 1 and 2, observed at 56 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)
      Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)
      All lanes : Anti-STK3 antibody [EP1466Y] (ab52641) at 1/50000 dilution (purified)

      Lane 1 : C6 whole cell lysate
      Lane 2 : NIH/3T3 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)
      Western blot - Anti-STK3 antibody [EP1466Y] (ab52641)
      All lanes : Anti-STK3 antibody [EP1466Y] (ab52641) at 1/50000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    文献

    This product has been referenced in:
    • Papaspyropoulos A  et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73. Nat Commun 9:424 (2018). Read more (PubMed: 29382819) »
    • Yang Z  et al. miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells. Biochim Biophys Acta 1852:1477-89 (2015). Read more (PubMed: 25892184) »

    See all 13 Publications for this product

    发表研究结果有使用 ab52641？请让我们知道，以便我们可以引用本数据表中的参考文章。

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