概述

  • 产品名称Anti-STK3抗体
    参阅全部 STK3 一抗
  • 描述
    小鼠单克隆抗体to STK3
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Cow, Dog, Xenopus laevis, Chimpanzee, Macaque Monkey, Rhesus monkey, Orangutan, Zebra finch
  • 免疫原

    Recombinant fragment corresponding to amino acids 253-350 (

    DFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRH EEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTM

    ) of Human STK3 (NP_006272) with a proprietary tag

  • 阳性对照
    • Raw 264.7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab89773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.

靶标

  • 功能Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
  • 组织特异性Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • 细胞定位Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610042I06Rik antibody
    • EC 2.7.11.1 antibody
    • FLJ90748 antibody
    • Kinase responsive to stress 1 antibody
    • KRS1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • Mammalian STE20-like protein kinase 2 antibody
    • Mammalian sterile 20-like 2 antibody
    • Mess1 antibody
    • MST antibody
    • MST-2 antibody
    • MST2 antibody
    • Mst3 antibody
    • Serine/threonine kinase 3 (STE20 homolog, yeast) antibody
    • Serine/threonine kinase 3 (Ste20, yeast homolog) antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase Krs1 antibody
    • Serine/threonine-protein kinase 3 antibody
    • Serine/threonine-protein kinase Krs-1 antibody
    • STE20 like kinase MST2 antibody
    • STE20-like kinase MST2 antibody
    • Stk3 antibody
    • STK3_HUMAN antibody
    • wu:fc19e11 antibody
    • zgc:55383 antibody
    see all

Anti-STK3 antibody 图像



  • Predicted band size : 56 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: STK3 knockout HAP1 cell lysate (20 µg)

    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: 293T cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab89773 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab89773 was shown to specifically react with STK3 when STK3 knockout samples were used. Wild-type and STK3 knockout samples were subjected to SDS-PAGE. ab89773 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-STK3 antibody (ab89773) at 1 µg/ml + immunogen (98aa protein fragment with a tag of 26kDa) at 0.2 µg

    Predicted band size : 56 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-STK3 antibody (ab89773) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 56 kDa

Anti-STK3 antibody (ab89773)参考文献

ab89773 has not yet been referenced specifically in any publications.

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