概述

  • 产品名称Anti-STIP1抗体
    参阅全部 STIP1 一抗
  • 描述
    兔多克隆抗体to STIP1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: QRCMMAQYNR HDSPEDVKRR AMADPEVQQI MSDPAMRLIL EQMQKDPQAL, corresponding to a region within C terminal amino acids 469-518 of Human STIP1

  • 阳性对照
    • Human muscle. Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab65046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-STIP1 antibody 图像

  • Anti-STIP1 antibody (ab65046) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling STIP1 with ab65046 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-STIP1 antibody (ab65046)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab65046.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"