概述

  • 产品名称Anti-STIM2抗体
    参阅全部 STIM2 一抗
  • 描述
    兔多克隆抗体to STIM2
  • 特异性ab59342 is predicted to have no cross-reactivity to STIM1.
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    An 18 amino acid peptide from near the carboxy terminus of human STIM2.

  • 阳性对照
    • A20 cell lysate. IF: HeLa cell line

性能

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应用

Our Abpromise guarantee covers the use of ab59342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 84 kDa).Can be blocked with STIM2 peptide (ab176417).
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • 组织特异性Expressed in all tissues and tumor cell lines examined.
  • 序列相似性Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Glycosylated.
    Phosphorylated predominantly on Ser residues.
  • 细胞定位Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ39527 antibody
    • KIAA1482 antibody
    • STIM 2 antibody
    • STIM2 antibody
    • STIM2_HUMAN antibody
    • Stromal interaction molecule 2 antibody
    see all

Anti-STIM2 antibody 图像

  • Lane 1 : Anti-STIM2 antibody (ab59342) at 0.5 µg/ml
    Lane 2 : Anti-STIM2 antibody (ab59342) at 1 µg/ml
    Lane 3 : Anti-STIM2 antibody (ab59342) at 2 µg/ml

    Lane 1 : A-20 cell lysate
    Lane 2 : A-20 cell lysate
    Lane 3 : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 84 kDa
  • ICC/IF image of ab59342 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59342, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-STIM2 antibody (ab59342)参考文献

This product has been referenced in:
  • Bréchard S  et al. STIM1 but not STIM2 is an essential regulator of Ca2+ influx-mediated NADPH oxidase activity in neutrophil-like HL-60 cells. Biochem Pharmacol 78:504-13 (2009). Read more (PubMed: 19433064) »
  • Chiu TY  et al. Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead ions. Toxicol Sci 110:353-62 (2009). Read more (PubMed: 19429662) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (microsomal fraction)
Loading amount 30 µg
Specification microsomal fraction
Gel Running Conditions Reduced Denaturing (bis-tris 4-20% gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (microsomal fraction)
Loading amount 20 µg
Specification microsomal fraction
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (affinity purified)
Specification affinity purified
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type Direct
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Abcam user community

Verified customer

提交于 Sep 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse skeletal muscle)
Loading amount 30 µg
Specification mouse skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Abcam user community

Verified customer

提交于 Aug 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells with transfected human stim2)
Loading amount 20 µg
Specification 293 cells with transfected human stim2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Aug 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"