概述

  • 产品名称
  • 描述
    兔多克隆抗体to STIL
  • 经测试应用
    适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Gorilla, Opossum, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1237 and 1287 of Human STIL (NP_003026.2).

  • 阳性对照
    • HeLa whole cell lysate. IF: T24/83 cell line

性能

应用

Our Abpromise guarantee covers the use of ab89314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Play a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
  • 组织特异性
    Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
  • 疾病相关
    Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
    Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
  • 翻译后修饰
    Phosphorylated following the activation of the mitotic checkpoint.
  • 细胞定位
    Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686O09161 antibody
    • MCPH7 antibody
    • OTTHUMP00000009566 antibody
    • SCL interrupting locus protein antibody
    • SCL-interrupting locus protein antibody
    • SCL/TAL1 interrupting locus antibody
    • SIL antibody
    • STIL antibody
    • STIL_HUMAN antibody
    • TAL 1 interrupting locus protein antibody
    • TAL-1-interrupting locus protein antibody
    see all

图片

  • All lanes : Anti-STIL antibody (ab89314) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 143 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of STIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 30 seconds.
  • ICC/IF image of ab89314 stained T24/83 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Xu X  et al. DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6. Nat Commun 8:15164 (2017). WB, ICC/IF ; Human . Read more (PubMed: 28447620) »
  • Bauer M  et al. Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging. EMBO J 35:2152-2166 (2016). Read more (PubMed: 27539480) »

See all 9 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam. I am sorry that you are having issues with ab89314 in western blot. Having looked over the data and protocol information you kindly sent, I have a few suggestions that may help: 1 - Although the p...

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