Anti-Steroidogenic Factor 1抗体(ab65815)

概述

  • 产品名称Anti-Steroidogenic Factor 1抗体
    参阅全部 Steroidogenic Factor 1 一抗
  • 描述
    兔多克隆抗体to Steroidogenic Factor 1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Steroidogenic Factor 1.

    (Peptide available as ab74765.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Testis; Ovary; Thymus.

性能

应用

Our Abpromise guarantee covers the use of ab65815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
  • 疾病相关Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
    Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
    Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻译后修饰Acetylation stimulates the transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AD4BP antibody
    • Adrenal 4 binding protein antibody
    • Adrenal 4-binding protein antibody
    • ELP antibody
    • FTZ 1 antibody
    • FTZ1 antibody
    • FTZF 1 antibody
    • FTZF1 antibody
    • Fushi tarazu factor (Drosophila) homolog 1 antibody
    • Fushi tarazu factor homolog 1 antibody
    • NR5A1 antibody
    • Nuclear receptor AdBP4 antibody
    • Nuclear receptor subfamily 5 group A member 1 antibody
    • POF7 antibody
    • SF 1 antibody
    • SF-1 antibody
    • SF1 antibody
    • SPGF8 antibody
    • SRXY3 antibody
    • Steroid hormone receptor Ad4BP antibody
    • Steroidogenic factor 1 antibody
    • Steroidogenic factor 1 nuclear receptor antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    • STF1_HUMAN antibody
    see all

Anti-Steroidogenic Factor 1 antibody 图像

  • All lanes : Anti-Steroidogenic Factor 1 antibody (ab65815) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

  • Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 10 seconds

    Image courtesy of Chun Fui Lai by Abreview.

    See Abreview

  • IHC-P image of Steroidogenic Factor 1 (ab65815) on Human testis sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.

    See Abreview

Anti-Steroidogenic Factor 1 antibody (ab65815)参考文献

This product has been referenced in:
  • El Hajj Chehadeh S  et al. Early methyl donor deficiency alters cAMP signaling pathway and neurosteroidogenesis in the cerebellum of female rat pups. Am J Physiol Endocrinol Metab 307:E1009-19 (2014). Read more (PubMed: 25294213) »

See 1 Publication for this product

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jul 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra, pH 6.0
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (COS-1 cells)
Loading amount 20 µg
Specification COS-1 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Chun Fui Lai

Verified customer

提交于 Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"