Anti-Steroidogenic Factor 1抗体(ab52928)

概述

  • 产品名称
    Anti-Steroidogenic Factor 1抗体
    参阅全部 Steroidogenic Factor 1 一抗
  • 描述
    小鼠多克隆抗体to Steroidogenic Factor 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Sheep, Horse, Cow, Human, Pig
  • 免疫原

    Recombinant fragment: KGFFKRTVQN NKHYTCTESQ SCKIDKTQRK RCPFCRFQKC LTVGMRLEAV RADRMRGGRN KFGPMYKRDR ALKQQKKAQI RANGFKLETG PPMGVPPPPP , corresponding to internal sequence amino acids 34-133 of Mouse Steroidogenic Factor 1

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 52 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
  • 疾病相关
    Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
    Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
    Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻译后修饰
    Acetylation stimulates the transcriptional activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AD4BP antibody
    • Adrenal 4 binding protein antibody
    • Adrenal 4-binding protein antibody
    • ELP antibody
    • FTZ 1 antibody
    • FTZ1 antibody
    • FTZF 1 antibody
    • FTZF1 antibody
    • Fushi tarazu factor (Drosophila) homolog 1 antibody
    • Fushi tarazu factor homolog 1 antibody
    • hSF 1 antibody
    • NR5A1 antibody
    • Nuclear receptor AdBP4 antibody
    • Nuclear receptor subfamily 5 group A member 1 antibody
    • POF7 antibody
    • SF 1 antibody
    • SF-1 antibody
    • SF1 antibody
    • SPGF8 antibody
    • SRXY3 antibody
    • Steroid hormone receptor Ad4BP antibody
    • Steroidogenic factor 1 antibody
    • Steroidogenic factor 1 nuclear receptor antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    • STF1_HUMAN antibody
    see all

实验方案

文献

ab52928 has not yet been referenced specifically in any publications.

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